Table II.

Oligonucleotide Primers used for isoform-selective RT-PCR of human AKR1 CDNA's

Isoform	Primer	Primer Sequence
AKR1C1	5′-primer	5′-CCAGCCATGGATTCGAAATAT-3′
	3′-primer	3′-CCATGTTAATATTCATCAGAG-5′
AKR1C2	5′-primer	5′-ACAGCCATGGATTCGAAATAC-3′
	3′-primer	3′-TCCATGTTAATATTCATCAGAA-5′
AKR1C3	5′-primer	5′-CAGGCCATGGATTCCAAACAG-3′
	3′-primer	3′-TCCATGTTAATATTCATCTGAAT-5′
AKR1C4	5′-primer	5′-CCATGGATCCCAAATATCAGC-3′
	3′-primer	3′-TCTATGCTAATATTCATCTGAA-5′
AKR1A1	5′-primer	5′-GGGGGCCATGGCGGTTCCTG-3′
	3′-primer	3′-GGGAAATTACTGGGCARGACTCTG-5′

NcoI-engineered start codons are in bold; stop-codons are underlined