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. Author manuscript; available in PMC: 2008 Oct 6.

Published in final edited form as: Methods Enzymol. 2004;378:31–67. doi: 10.1016/S0076-6879(04)78003-9

Table 5.

NP-1,2-dione and BP-7,8-dione mutate p53 under redoc-cycling conditions

Treatment	NP-1,2-dione			BP-7,8-dione

	Colonies			Colonies

	total	red	% red	total	red	% red
^aquinone alone	922	11	1.2	3380	32	0.9
quinone + NADPH	896	13	1.5	3160	31	1.0

^bμM quinone + NADPH + CuCl₂

0 μM	798	16	2.0	3290	34	1.0
0.031 μM	812	31	3.8	3284	41	1.2
0.0625 μM	1232	54	4.4	3080	51	1.7
0.125 μM	756	46	6.1	2536	69	2.1
0.25 μM	484	45	9.3	2200	113	5.1

^a

p53 mutagenesis was determined in the presence of 20 μM PAH o-quinone alone

^b

p53 mutagenesis was determined in the presence of increasing PAH o-quinone concentration in the presence of 1 mM NADPH and 100 μM CuCl₂