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Film cross-linking affects cell morphology during differentiation. Phase contrast microscopy observations of C2C12 cells differentiation cultured in DM on (PLL/HA)12 films cross-linked at various EDC concentrations at day 1 (left), day 3 (middle) and day 6 (right), respectively: EDC30, 300 kPa (A, B, C), EDC50, 354 kPa (D, E, F), EDC100, 382 kPa (G, H, I) and tissue culture polystyrene, P (J, K, L) (Scale bar is 100 μm).
