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Film cross-linking affects myotubes morphology. Immuno-fluorescence images of troponin T (green) and DNA (blue) of C2C12 cells cultured on (PLL/HA)12 films cross-linked at various EDC concentration after 3 days in DM: EDC30 (A), EDC50 (B), EDC100 (C), as compared to tissue-culture plastic (D). (Scale bar is 100 μm)
