Table 2.

Effect of dbcAMP and/or TFP or a sequential incubation of a combination of both on polarity of the cells

	Ratio [BC/100 cells]
Control	10.2  ± 1.5
DbcAMP	21.3  ± 0.5
TFP	9.5  ± 1.0
DbcAMP + TFP	10.8  ± 0.5
DbcAMP → TFP	19.2  ± 1.0
TFP → dbcAMP	9.7  ± 1.5

For incubation schemes see text. After the incubations, the cells were fixated and permeabilized with −20°C ethanol for 10 s, rehydrated in HBSS, and incubated with TRITC-labeled phalloidin and Hoechst to determine the ratio [BC/100 cells] (cf. Zegers et al., 1997). Data are presented as mean ± SEM of two or three independent experiments.