Figure 2.

Dynamin localization unaffected in an unc-104 worm. An unc-104 mutant animal was double labeled with anti-synaptotagmin (A) and anti-dynamin antibody (B). The anti-synaptotagmin antibody showed diffuse immunofluorescence in the head and concentrated immunofluorescence in the cell bodies of the ventral nerve cord (vc), which reflects the mislocalization of synaptic vesicles in unc-104 mutants. The anti-dynamin antibody showed immunofluorescence concentrated in the nerve ring (nr) and evenly distributed immunofluorescence along the ventral nerve cord (vc), similar to wild-type localization. The staining in the gut is mostly nonspecific, because it can also be detected in control experiments leaving out primary antibody (our unpublished results). In wild-type worms, which are shown here as a control, anti-synaptotagmin and anti-dynamin staining are both concentrated in the nerve ring (insets).