Figure 1.

Localization of rab11 and the Golgi marker α2,6-ST in BHK cells. Myc epitope-tagged ST was transiently overexpressed in BHK cells under the control of a constitutive CMV promoter together with GFP-tagged wild-type rab11 (A–C) or rab11S25N (D–F). At 17 h after transfection, cells were incubated at 20°C for 2 h to increase the ST signal in the Golgi (Ma et al., 1997). Cells were then fixed and processed for confocal microscopy. GFP-tagged rab11 proteins were visualized directly (A and D), whereas ST was detected using a mouse anti-myc antibody as the primary antibody and a horse anti-mouse, Texas Red–conjugated secondary antibody (B and E). Each image represents a single 0.4-μm section with ST and GFP-tagged rab11 proteins viewed in the same focal plane. (C and F) Merged images.