Figure 2.

Localization of rab11 and transferrin receptor in BHK cells. BHK cells were cotransfected with human transferrin receptor and GFP-tagged wild-type rab11 (A–C) or rab11S25N (D–F). At 17 h after transfection, cells were fixed and processed for confocal microscopy. Transferrin receptor was visualized using a goat anti-transferrin receptor antibody as the primary antibody and a biotinylated horse anti-goat antibody and Texas Red–conjugated avidin for detection purposes (A and D). GFP-tagged rab11 proteins were visualized directly (B and E). Each image represents a single 0.4-μm section with transferrin receptor and GFP-tagged rab11 proteins viewed in the same focal plane. (C and F) Merged images. The arrows in B designate some of the peripheral endosomes positive for wild-type rab11 and transferrin receptor.