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. 1999 Oct;10(10):3473–3488. doi: 10.1091/mbc.10.10.3473

Figure 8.

Figure 8

N-myristoylation is required for the association of p22 with microtubules. (A) p22-myr (12 μg), p22-rec (12 μg), and/or rat liver cytosol (500 μg) were incubated with purified tubulin (0.4 mg/ml) in the presence of PEM buffer and taxol, and processed as described in Figure 4. Equal amounts of supernatants (lanes 1–5) and microtubule pellets (lanes 7–11) were subjected to SDS-PAGE and Coomassie blue staining. Arrows indicates the position of p22 and tubulin. In lane 6, the molecular weights in kDa are as follows: 97, 45, 31, 21, 14. (B) N-myristoy-lated p22-E134A (12 μg) and/or rat liver cytosol (500 μg) were incubated with purified tubulin (0.4 mg/ml) in the presence of PEM buffer and taxol, and processed as described in Figure 4. Equal amounts of supernatants (lanes 1–3) and microtubule pellets (lanes 5–7) were subjected to SDS-PAGE and Coomassie blue staining. In lane 4, the molecular weights in kDa are as follow: 97, 45, 31, 21, 14. Arrows indicates the position of p22 and tubulin.