Figure 10.

FAK-dependent relocalization of pp60^c-src to focal adhesions. CE cells (expressing no exogenous protein) were fixed, permeabilized, and stained with monoclonal antibody EC10 to detect endogenous Src (A) or with BC4 to detect endogenous FAK (B). Rhodamine-conjugated goat anti-mouse secondary antibodies were used to detect the primary mAb, and fluorescein-conjugated donkey anti-rabbit secondary antibodies were used to detect BC4. Cells expressing pRL c-src (C) or pRL c-src + RCAS B FAK (D) were stained with EC10 to detect Src. CE cells expressing RCAS B c-src + RCAS A FAK (E and F) were stained with BC4 to examine FAK localization (E) and with EC10 to detect the localization of exogenously expressed c-src (F).