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. 1999 Oct;10(10):3489–3505. doi: 10.1091/mbc.10.10.3489

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Copyright © 1999, The American Society for Cell Biology

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Tyrosine phosphorylation of paxillin in cells coexpressing FAK variants and Src or fyn. Paxillin was immunoprecipitated from cell lysates and Western blotted for phosphotyrosine (top panels) and for paxillin (bottom panels). (A) Paxillin was immunoprecipitated from lysates of CE cells (lane 1) or CE cells expressing RCAS B fyn (lane 2), RCAS A FAK (lane 3), RCAS A FAK + RCAS B fyn (lane 4), RCAS A FAK^576/577FF (lane 5), or RCAS A FAK^576/577FF + RCAS B fyn (lane 6). (B) Paxillin was immunoprecipitated from lysates of CE cells (lane 1) or CE cells expressing RCAS B fyn (lane 2), RCAS A FAK (lane 3), RCAS A FAK + RCAS B fyn (lane 4), RCAS A FAK^397F (lane 5), RCAS A FAK^397F + RCAS B fyn (lane 6), RCAS A FAK^454R (lane 7), RCAS A FAK^454R + RCAS B fyn (lane 8), RCAS A FAK^454R/397F (lane 9), or RCAS A FAK^454R/397F + RCAS B fyn (lane 10). (C) Paxillin was immunoprecipitated from lysates of CE cells (lane 1) or CE cells expressing RCAS A FAK (lane 2), RCAS B Src (lane 3), RCAS A FAK + RCAS B Src (lane 4), RCAS A FAK^397F + RCAS B Src (lane 5), RCAS A FAK^576/577FF + RCAS B fyn (lane 6), or RCAS A FAK^454R + RCAS B fyn (lane 7). (D–F) Twenty-five micrograms of lysate were blotted for FAK, Src, or fyn.