Figure 1. Mcl-1 expression in different T cell populations as assessed by intracellular staining.

A. Expression of Mcl-1 (thick lines) in double-negative CD4⁻CD8⁻CD3⁻ (DN), double-positive CD4⁺CD8⁺ (DP), CD4 single-positive CD4⁺CD8⁻TCRβ^hi (CD4SP), CD8 single-positive CD4⁻CD8⁺TCRβ^hi (CD8SP), immature single-positive CD4⁻CD8⁺TCRβ ⁻ (ISP), and positively selected DP CD4⁺CD8⁺CD69⁺TCRβ^hi thymocytes as assessed by FACS. B. Expression of Mcl-1 (thick lines) in different DN subpopulations: DN1 – CD44⁺CD25⁻ DN2 – CD44⁺CD25⁺; DN3 – CD44⁻CD25⁺; DN4 – CD44⁻CD25⁻ as assessed by FACS. C. Expression of Mcl-1 (thick lines) in peripheral T cells: naïve (CD44⁻CD62L^hi) CD4⁺ or CD8⁺ T cells; activated/effector memory (CD44⁺CD62L^lo) CD4⁺ or CD8⁺ T cells and central memory (CD44⁺CD62L^hi) CD8⁺ T cells as assessed by FACS. (A–C) The shaded histograms depict isotype control staining. For DP, CD4SP, CD8SP, and positively selected DP cells the thin lines represent staining for Mcl-1 in these cells from Mcl-1^f/fCD4-Cre mice. The numbers represent the ratios of the fluorescence intensity of Mcl-1 staining divided by the fluorescent intensity of the isotype control staining. Data are representative of four individual experiments.