Table 1.

Electron Transport Activity of Monomeric Control and Pressurized Cytochrome c Oxidase^a

	CcO-A	CcO-B	CcO-C
control	350	340	N/A
2.5 kbar	315	290	147

^aActivities expressed as micromoles of ferrocytochrome c oxidized per micromole of CcO per second. CcO was pressurized at 2.5 kbar for 2 h at 25 °C. CcO, before and after being exposed to hydrostatic pressure, was resolved into CcO-A, CcO-B, and CcO-C with a HiTrapQ anion-exchange column (refer to Figures 3 and 6) and the electron transport activity of each form of CcO measured spectrophotometrically as described in Experimental Procedures. The standard error of the mean for activities was ±3% for control CcO and ±5% for pressurized CcO.