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. 1999 Oct;10(10):3507–3520. doi: 10.1091/mbc.10.10.3507

Figure 2.

Figure 2

The motility defect in the F56 and V3 strains segregates with the defect in the LC2 gene. (A) The initial F56 isolate was crossed to 137c, and a slow-swimming offspring was isolated and crossed again to 137c. Twenty-five tetrads were dissected from this cross and scored for motility. The Oda phenotype segregated 2:2. DNA was isolated from the four products of one full tetrad and a single product of 11 additional tetrads, cut with PstI, and analyzed by Southern blotting using the LC2 cDNA as the probe. In every case, the Oda− phenotype segregated with the deletion revealed by the LC2 cDNA, indicating that the motility defect is tightly linked to this deletion mutation. (B) V3 was crossed to H8− (Wild Type), and the resultant tetrads were dissected. DNA was isolated from the parental strains, the four products of one tetrad, and a single product of 15 additional tetrads, cut with PvuII, and analyzed by Southern blotting with the LC2 cDNA as the probe. Again, the Oda− phenotype segregated with the deletion revealed by the LC2 cDNA.