Table I.
Ligands used to investigate the binding response of HGF by SPR.
| Immobilised ligands in SPR | Source/product number | Code | Goal of investigation |
|---|---|---|---|
| Monoclonal anti-HGF AB | R&D Systems/RDS MAB294 | MN | Determine amount of HGF |
| Recombinant HGF receptor (c-met)/fc chimera | R&D Systems/358 MT | c-Met | Analyze HGF binding to c-met receptor |
| HS proteoglycan | Sigma-Aldrich/H4777 | HSPG | Analyze HGF binding to HSPG |
| Polyclonal anti-HGF AB, affinity isolated | Sigma-Aldrich/HH0652 | PK | Determine amount of HGF |
| H-170 rabbit polyclonal AB | Santa Cruz/sc-13087 | H-170 | Bind amino acids 1–170 of human HGFβ |
| N-19 affinity-purified goat polyclonal AB | Santa Cruz/sc-1356 | N-19 | Peptide mapping at the N-terminus of human HGFβ |
| N-17 affinity-purified goat polyclonal AB | Santa Cruz/sc-1357 | N-17 | Peptide mapping at the N-terminus of human HGFα |
| C-20 affinity-purified goat polyclonal AB | Santa Cruz/sc-1358 | C-20 | Peptide mapping at the C-terminus of human HGFα |
| H-145 rabbit polyclonal AB | Santa Cruz/sc-7949 | H-145 | Bind amino acids 32–176 of human HGFα |
| D-19 goat polyclonal IgG | Santa Cruz/sc-34461 | D19 | |
| Epitope mapping in an internal region of human HGFβ |
All ligands were diluted (1:10 in 10 mM acetate buffer, pH 4.5) prior to immobilisation. AB, antibody.