Table V.

Binding of recombinant HGF (R&D carrier-free GJ174034, diluted in PBS) to ligands, showing degradation over time.

Samples containing HGF	C-met (22377 RU)	MN (19820 RU)	HSPG (14337 RU)
2.5 μg/ml freshly reconstituted	194	1260	95
After 34 min + PK (10 μl)	1	−1	−19
2.5 μg/ml after 15 h room temperature	181	373	147
2.5 μg/ml after 15 h, −20°C and thawed	94	283	45
Negative control PBS	−10	−8	−24
Positive control after 15 h	403	508	512

The results are obtained from Biacore runs performed on the same chip. The immobilisation levels (RU) are mentioned in parentheses.