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Analysis of HGF in buffy coat of blood donor by SPR (RU) and ELISA (ng/ml).
| HSPG | c-Met | N-17 | D19 | ELISA (dilution 1:10) | |
|---|---|---|---|---|---|
| Buffy coat centrifuged 3000g for 20 min, diluted 1:20 in PBS | 35 | 247 | 272 | 335 | 3.7 |
| Serum from same person, diluted 1:20 in PBS | 38 | 317 | 336 | 330 | 2.3 |
| Haemolysed buffy coat, frozen at -70°C, thawed, centrifuged at 3000g for 20 min, diluted in 1:20 PBS | 15 | 163 | 99 | 118 | 5.7 |
Statistical analysis of results could not be performed because there were too few observations.
