TABLE 2.

The contribution of glyceroneogenesis and glycolysis to triglyceride glycerol synthesis in adipose tissue Values are the mean ± S.E.; range is indicated in parentheses. GlyNG, glyceroneogenesis; Suc Sup + Glc Inf, sucrose-supplemented animals infused with glucose during tracer study. Glyceroneogenesis was quantified using tritium, [³H₂]O, incorporation into triglyceride glycerol and is expressed as nmol of pyruvate/g/h. The contribution of glucose was quantified using [U-¹⁴C]glucose and is expressed as three carbon equivalents. Total, includes carbon coming directly from glucose plus indirectly via lactate. Direct, incorporation of glucose into triglycerides via triosephosphate.

	Epididymal fat				Mesenteric fat
	TG	GlyNG	Glycolysis		TG	GlyNG	Glycolysis
			Total	Direct			Total	Direct
	μmol/g	nmol/g/h	nmol/g/h			μmol/g	nmol/g/h	nmol/g/h
Control (n = 5)	400 ± 21.6	604 ± 56.6	294 ± 37.9	82.3 ± 12.5	414 ± 17.7	789 ± 70.5	362 ± 45.0	86.3 ± 13.7
48-h fast (n = 5)	352 ± 34.4	511 ± 65.0	66.3 ± 5.6a	(–24.5–11.5)b	359 ± 45.9	816 ± 96.6	94.8 ± 6.63vb	(–42.0–10.5)a
Suc Sup + Glc Inf (n = 5)	723 ± 19.2b	1022 ± 57.1b	671 ± 67.9a	149 ± 19.3	695 ± 11.7a	1410 ± 85.2b	1111 ± 133a	277 ± 34.1a

^ap < 0.05 vs. control

^bp < 0.01 vs. control