Mapping the SAGA-dependent region of RNR3. A,
lacZ-containing reporter vectors were constructed by fusing the upstream
and core promoter regions of RNR3 and RPS5 together as
indicated in the panel. The sequences within each of the genes are indicated
below and are relative to the start site of transcription, which was
set to +1. Gray bars indicate the approximate locations of the damage
response elements. B, transcription was measured by Northern blotting
for lacZ message as described in Li and Reese
(24), and a representative
Northern blot is shown. WT, wild type. C, quantification of
lacZ signals corrected for scR1 levels. The level of
lacZ in untreated wild type cells was set to 1.0. Results are
presented as the means and S.D. of three experiments. The values shown in
gray and black bars were calculated from untreated and
MMS-treated samples, respectively. D, as in C, except that
the data from untreated samples are shown.