FIGURE 5.

Expression ofα2 integrin constructs in HRA-19, human colorectal cancer cells. A, wild-type α2 and chimeric α2α1 integrin constructs transfected into HRA-19 cells. B, α2 integrin expression in α2 and α2α1 transfectants and HRA-19 cells. The experiment was performed twice. C, α2α1 integrin expression. α2α1 integrin was immunoprecipitated using an α1 cytoplasmic domain antibody, then detected using Western blot with an α2 extracellular domain antibody. The chimeric protein band was found only in α2α1-transfected colonies, α2α1β and α2α1E. The experiment was performed five times. D, α2 integrin localization was examined by immunofluorescence in α2F, HRA-19, and α2α1E cells. Bar, 100 μm.