FIGURE 3.

An inhibitor of NF-κB activation blocks co-translocation of myotrophin and p65. A, neonatal rat ventricular myocytes were pretreated with PDTC for 30 min prior to myotrophin treatment. p65 (red) was observed in the cytoplasm. B, when cells were treated with Alexa 488 labeled wild type myotrophin (green) without PDTC pretreatment, nuclear translocation of both myotrophin (green) and p65 (red) was observed. C, this translocation was inhibited when cells were pretreated with PDTC before treatment with myotrophin. Co-localization of myotrophin and p65 was evident from yellow fluorescence. The nucleus is stained with DAPI (blue).