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Effect of mutants of hairpin loop on myocyte protein synthesis. Compared with the MBP-wild type myotrophin fusion, incorporation of 3[H]leucine into neonatal rat ventricular myocytes was suppressed in cells treated with MBP-myotrophin mutants at glutamic acid 33 (E33A), arginine 36 (R36A), aspartic acid 65 (D65A), and lysine 66 (K66A), but not in histidine 68 (H68A). The data show the means (± S.E.) of three independent sets of results.
