FIGURE 8.

In vitro interaction of myotrophin and NF-κB-p65. A, MBP-wild type/E33A mutant myotrophin incubated with amylose resin in equimolar (15 pmol) ratio for overnight at 4 °C, and amylose-bound protein was resolved in 10% SDS-PAGE and immunoblotted (IB) for NF-κB-p65 (upper panel). Immunoblotting for myotrophin as loading control (lower panel). B, band intensity for immunoblotting. NF-κB-p65 from three independent experiments was quantified using ImageJ software and expressed in arbitrary units.