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NO donors activate AMPK in HUVECs. HUVECs were exposed to structurally unrelated NO donors (50 μm) for one half-life accordingly. Cell lysates were subjected to Western blot analysis using an antibody against phospho-AMPK (Thr-172), phospho-eNOS (Ser-1177), AMPK-α, eNOS, or anti-β-actin for counterstaining. Representative blots and densitometric analysis are shown (mean ± S.E., n >= 3). *, p < 0.05 versus vehicle.
