FIGURE 9.

AMPK is involved in endogenous NO production. A and B, HUVECs were pretreated with compound C (compC, 20 μm, 30 min) prior to stimulation with SNP (50 μm, 1 h). Cell lysates were subjected to Western blot analysis using antibodies against phospho-AMPK (Thr-172), AMPK-α, phospho-eNOS (Ser-1177), eNOS, or β-actin. Typical blots and densitometric analysis (means ± S.E., n >= 3) are presented. C, PTIO (0.3 mm, 30 min) or A23187 (2μm, 10 min) was added to primary cultured AMPK-α2 knock-out and WT mouse aorta endothelium cells, and NO production was measured as described under “Experimental Procedures.” *, p < 0.05 versus vehicle.