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. 2008 Oct 10;283(41):27628–27635. doi: 10.1074/jbc.M805711200

FIGURE 3.

FIGURE 3.

A, effects of overexpression of SIRT1 on AMPK and ACC phosphorylation. Cells grown in 6-well plates were transfected with 2 μg of wild type SIRT1 or the inactive SIRT1 H355Y mutant. The ratios of phosphorylated/total AMPK and ACC were increased by SIRT1 overexpression, whereas SIRT1 H355Y had no effects (*, p < 0.05 versus pcDNA, n = 3). A similar increase in AMPK phosphorylation was observed in other experiments. B, effects of SIRT1 knockdown on AMPK and ACC phosphorylation in HEK293T cells. Transfection of HEK293T cells with siRNA targeting SIRT1 decreased a ratio of phosphorylated/total of AMPK and ACC (*, p < 0.05 versus siRNA control, n = 6). C, effects of SIRT1 knockdown on MARK1 phosphorylation in HepG2 cells. Lentiviral infection of shRNA targeting sirt1 in HepG2 cells decreased the ratio of phosphorylated to total MARK1 by 69% (*, p <0.05, n = 6).