FIGURE 4.

A–C, effects of starvation on hepatic LKB1 activity (A), acetylation (B), and AMPK phosphorylation (C). Sprague-Dawley rats were starved for 48 h or starved and then refed standard chow for 24 h (Fed). Under pentobarbital anesthesia, the livers were removed and then freeze-clamped for the indicated measurements. After 48 h of starvation, LKB1 activity and the phosphorylation of AMPK were higher, and LKB1 acetylation was lower that in the starved-refed rats (^*, p < 0.05 versus fed, n = 6).