FIGURE 7.

Isoflavone-induced apoptosis. C4-2B and LNCaP PCa cells were treated with 100 ng/ml IGF-1, 50 μm isoflavone, or 50 μm isoflavone plus 100 ng/ml IGF-1 for 48 h. Apoptotic cell death was assessed by Hoechst staining (A; arrow, condensed and granular stained nuclei in apoptotic cells), cell death detection enzyme-linked immunosorbent assay (B;^*, p < 0.05, compared with control; n = 3), caspase-3 activity assay (C;^*, p < 0.05, compared with control; n = 3), and Western blot analysis for cleavage of caspase-3 (D).