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. 1999 Oct;10(10):3521–3538. doi: 10.1091/mbc.10.10.3521

Figure 3.

Figure 3

Suppression of arp2-2 actin and endocytosis phenotypes by LAS17. Strain YMW221U (bearing the arp2-2:URA3 ts allele) was transformed with vector (a), with pAMW171 (LAS17 2μ) (b), and with pAMW200 (ARP2 in pGAD) (c). (A) Transformants were grown at 25°C in liquid YPD to log phase and shifted to 37°C for 2 h. Cells were fixed, stained with TRITC-phalloidin, and photographed as described in MATERIALS AND METHODS. Bar, 5 μm. (B) Transformants were grown in liquid YPD at 25°C to log phase and incubated for 1 h with LY as described in MATERIALS AND METHODS. Cells were photographed using an FITC filter (LY) or Nomarski optics (differential interference contrast [DIC]).