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LAS17 allele-specific suppression of ARP3 ts mutants. The arp3Δ (YMW301T) strain rescued by five different ARP3 ts alleles (arp3-9 to arp3-13 and ARP3) and strain YMW321L (bearing the arp3-14: LEU2 allele) were transformed by vector (a), pYEW170 (LAS17, 2μ) (b), or pCMW304 rescue plasmid (ARP3:LEU2) (c). Freshly cultured transformants were spotted onto YNB − Leu and Ura plates, which were incubated at 25°C for 5 d or at 36°C for 3 d before being photographed.
