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. 1999 Oct;10(10):3521–3538. doi: 10.1091/mbc.10.10.3521

Figure 8.

Figure 8

Loss of Arp2p and Arp3p polarity in las17Δ strains. (A) Localization of Arp2-gfp in wild-type and las17Δ/las17Δ cells. One allele of ARP2 was replaced by an ARP2:EGFP (Cormack et al., 1997) allele in FY1679 (WT) and in strain YMW173K (las17Δ/las17Δ) as described in MATERIALS AND METHODS. Cells were grown at 25°C in YPD, harvested, resuspended in 1 M sorbitol, and immediately put on slides for microscopic examination. Images were recorded using a GFP filter. (B) Localization of Arp3-gfp in wild-type and las17Δ/las17Δ cells. Experimental conditions were as in A. Bar, 5 μm.