Table 1.

Plasmids used in this study

Plasmid	Description
pIMW18-U	pUC18 containing ARP2 tagged with URA3 (this study)
pIMW300	pUC18 containing ARP3 as an 1.85-kb BamH1 insert (this study)
pYCW204	pUN60, CENIV, URA3, SUP11, ARP2 (Moreau et al., 1996)
pCMW306	pUN60, CENIV, URA3, SUP11, ARP3, constructed by ligating the SalI–BamHI fragment of pIMW300 into the same sites of pUN60 (this study)
pCMW304	pRS314 (Sikorski and Hieter, 1989), ARP3∶LEU2 (this study)
pYCW207	pUN90, CENIV, HIS3, ARP2 (Moreau et al., 1996)
pYEW170	pFL44, 2μD, URA3, LAS17 and flanking regions as a 4-kb BamHl insert (this study)
pAS2	2μD, TRP1, ADHpro-NLS-GAL4BD, HA, CYH2, bait vector (Durfee et al., 1993)
pAMW172	pAS2, GAL4BD fused to full-length LAS17 (this study)
pDAB7	pAS2 derived, CEN, GAL4BD fused to full-length ACT1 (Amberg et al., 1995)
pACT2	2μD, LEU2, ADHpro-NLS-GAL4AD, HA, prey vector (Durfee et al., 1993)
pAMW253	pACT2, GAL4AD fused to intronless full length ARP2 (this study)
pAMW315	pACT2, GAL4AD fused to full length ARP3 (this study)
pAMW173	pACT2, GAL4AD fused to full length LAS17 (this study)
PGAD.GH	2μD, LEU2, ADHpro-NLS-GAL4AD, prey vector (Chien et al., 1991)
pAMW200	pGAD.GH, ARP2 (this study)
pAMW171	PGAD.GH, LAS17 (2μ overexpression, this study)
pFA6a-kanMX4	pFA6a, cassette for resistance to G418 (Wach et al., 1994)
pFA6a-HIS3MX6	pFA6a, his3 complementing heterologous HIS5 ORF from S. pombe (Wach et al., 1997)
pFA6a GFPS65T-kanMX6	Same as pFA6a-kanMX4, containing GFP (S65T) upstream of the kanMX6 module (Wach et al., 1997)