Table 1.
Plasmids used in this study
| Plasmid | Description |
|---|---|
| pIMW18-U | pUC18 containing ARP2 tagged with URA3 (this study) |
| pIMW300 | pUC18 containing ARP3 as an 1.85-kb BamH1 insert (this study) |
| pYCW204 | pUN60, CENIV, URA3, SUP11, ARP2 (Moreau et al., 1996) |
| pCMW306 | pUN60, CENIV, URA3, SUP11, ARP3, constructed by ligating the SalI–BamHI fragment of pIMW300 into the same sites of pUN60 (this study) |
| pCMW304 | pRS314 (Sikorski and Hieter, 1989), ARP3∶LEU2 (this study) |
| pYCW207 | pUN90, CENIV, HIS3, ARP2 (Moreau et al., 1996) |
| pYEW170 | pFL44, 2μD, URA3, LAS17 and flanking regions as a 4-kb BamHl insert (this study) |
| pAS2 | 2μD, TRP1, ADHpro-NLS-GAL4BD, HA, CYH2, bait vector (Durfee et al., 1993) |
| pAMW172 | pAS2, GAL4BD fused to full-length LAS17 (this study) |
| pDAB7 | pAS2 derived, CEN, GAL4BD fused to full-length ACT1 (Amberg et al., 1995) |
| pACT2 | 2μD, LEU2, ADHpro-NLS-GAL4AD, HA, prey vector (Durfee et al., 1993) |
| pAMW253 | pACT2, GAL4AD fused to intronless full length ARP2 (this study) |
| pAMW315 | pACT2, GAL4AD fused to full length ARP3 (this study) |
| pAMW173 | pACT2, GAL4AD fused to full length LAS17 (this study) |
| PGAD.GH | 2μD, LEU2, ADHpro-NLS-GAL4AD, prey vector (Chien et al., 1991) |
| pAMW200 | pGAD.GH, ARP2 (this study) |
| pAMW171 | PGAD.GH, LAS17 (2μ overexpression, this study) |
| pFA6a-kanMX4 | pFA6a, cassette for resistance to G418 (Wach et al., 1994) |
| pFA6a-HIS3MX6 | pFA6a, his3 complementing heterologous HIS5 ORF from S. pombe (Wach et al., 1997) |
| pFA6a GFPS65T-kanMX6 | Same as pFA6a-kanMX4, containing GFP (S65T) upstream of the kanMX6 module (Wach et al., 1997) |