Table 2.
Yeast strains used in this study
| Strain | Genotype | Source |
|---|---|---|
| YPH499 | MATa, ade2-101, his3-Δ200, leu2-Δ1, lys2-801, trp1-Δ63, ura3-52 | P. Hieter |
| YPH500 | MATa , ade2-101, his3-Δ200, leu2-Δ1, lys2-801, trp1-Δ63, ura3-52 | P. Hieter |
| YPH501 | MATa, ade2-101, his3-Δ200, leu2-Δ1, lys2-801, trp1-Δ63, ura3-52 | P. Hieter |
| MATa, ade2-101, his3-Δ200, leu2-Δ1, lys2-801, trp1-Δ63, ura3-52 | ||
| YMW11 | MATa, arp2∷LEU 2, ade2-101, his3Δ200, leu2-Δ1, lys2-801, trp1-Δ63, ura3-52 (pYCW204) | Moreau et al. (1996) |
| YMW81 | MATa, arp2-1(H330L), ade2-101, his3-Δ200, leu2-Δ1, lys2-801, trp1-Δ63, ura3-52 | Moreau et al. (1996) |
| YMW201U | MATa, ARP2∶URA3, ade2-101, his3-Δ200, leu2-1, lys2-801, trp1-Δ63, ura3-52 | This study |
| YMW211U | MATa, arp2-1(H330L)∶URA3, ade2-101, his3-Δ200, leu2-Δ1, lys2-80, trp1-Δ63, ura3-52 | This study |
| YMW221U | MATa, arp2-2(G19D)∶URA3, ade2-101, his3-Δ200, leu2-Δ1, lys2-801, trp1-Δ63, ura3-52 | This study |
| YMW231U | MATa, arp2-3(E256K)∶URA3, ade2-101, his3-Δ200, leu2-Δ1, lys2-801, trp1-Δ63, ura3-52 | This study |
| YMW241U | MATa, arp2-4(L280P)∶URA3, ade2-101, his3-Δ200, leu2-Δ1, lys2-801, trp1-Δ63, ura3-52 | This study |
| YMW251U | MATa, arp2-5(L316S)∶URA3, ade2-101, his3-Δ200, leu2-Δ1, lys2-801, trp1-Δ63, ura3-52 | This study |
| YMW261U | MATa, arp2-6(G35S Y146N)∶URA3, ade2-101, his3-Δ200, leu2-Δ1, lys2-801, trp1-Δ63, ura3-52 | This study |
| YMW271U | MATa, arp2-7(F127S F203Y)∶URA3, ade2-101, his3-Δ200, leu2-Δ1, lys2-801, trp1-Δ63, ura3-52 | This study |
| YMW300T | MATa, arp3Δ∷TRP1, ade2-101, his3-Δ 200, leu2-Δ1, lys2-801, trp1-Δ63 ura3-52 | This study |
| MATa, ARP3, ade2-10, his3-Δ200, leu2-Δ1 lys2-801 trp1-Δ63 ura3-52 | ||
| YMW301T | MATa, arp3Δ∷TRP1 ade2-101 his3-Δ200, leu2-Δ1, lys2-801, trp1-Δ63, ura3-52 (+pCMW 306) | This study |
| YMW311L | MATa, ARP3∶LEU2, ade2-101, his3-Δ200, leu2-Δ1, lys2-801, trp1-Δ63, ura3-52 | This study |
| YMW321L | MATa, arp3-14 (P33L aa 48-108Δ)∶LEU2, ade2-101, his3-Δ200, leu2-Δ1, lys2-801, trp1-Δ63, ura3-52 | This study |
| YMW171K | MATa, las17Δ∷kanMX4, ade2-101, his3-Δ200, leu2-Δ1, lys2-801, trp1-Δ63, ura3-52 | This study |
| YMW172K | MATa, las17Δ∷kanMX4, ade2-101, his3-Δ200, leu2-Δ1, lys2-801, trp1-Δ63, ura3-52 | This study |
| YMW173K | MATa, las17Δ∷kanMX4, ade2-101, his3-Δ200, leu2-Δ1, lys2-801, trp1-Δ63, ura3-52 | This study |
| MATa, las17Δ∷kanMX4, ade2-101, his3-Δ200, leu2-Δ1, lys2-801, trp1-Δ63, ura3-52 | ||
| YMW175K | MATa, ura3-52, his3-Δ200, leu2-Δ1, arp2Δ∷LEU2, trp1-Δ63 LAS17-PROTA∶kanMX6 (pYEW248) | This study |
| RH4207 | MATa, las17Δ∷kanMX4, bar1∷LYS2, ade2-101, his3-Δ200, leu2-Δ1, trp1-Δ63, ura3-52 | This study |
| FY1679 | MATa, his3-Δ200, leu2-Δ1, trp1-Δ63, ura3-52, GAL2 | B. Dujon/F. Winston |
| MATa, HIS3, LEU2, TRP1, ura3-52, GAL2 | (EUROFAN strain) | |
| FDW23GK | MATa, ARP2-S65TGFP∶kanMX6, his3-Δ200, leu2-Δ1, trp1-Δ63, ura3-52, GAL2 | This study |
| MATa, ARP2-S65TGFP∶kanMX6, his3-Δ200, LEU2, TRP1, ura3-52, GAL2 | ||
| FDW24GK | MATa, ARP2, his3-Δ200, leu2-Δ1, trp1-Δ63, ura3-52 GAL2 | This study |
| MATa, ARP2-EGFP∶kanMX6, HIS3, LEU2, TRP1, ura3-52 GAL2 | ||
| FDW34GK | MATa, ARP3, his3-Δ200, leu2-Δ, 1 trp1-Δ63, ura3-52, GAL2 | This study |
| MATa, ARP3-EGFP∶kanMX6, HIS3, LEU2, TRP1, ura3-52, GAL2 | ||
| FMW17GK | MATa, LAS17-GFP∶kanMX4, his3-Δ200, leu2-Δ1, trp1-Δ63, ura3-52, GAL2 | This study |
| MATa, LAS17-GFP∶kanMX4, HIS3, LEU2, trp1-Δ63, ura3-52, GAL2 | ||
| FDW174H | MATa, las17Δ∷HISMX6, his3-Δ200, LEU2, TRP1, ura3-52, GAL2 | This study |
| MATa, las17Δ∷HISMX6, his3-Δ200, LEU2, TRP1, ura3-52, GAL2 | ||
| FDW180HK | MATa, las17Δ∷HISMX6, ARP2, his3-Δ200, LEU2, TRP1, ura3-52, GAL2 | This study |
| MATa, las17Δ∷HISMX6, ARP2-EGFP∶kanMX6, his3-Δ200, LEU2, TRP1, ura3-52, GAL2 | ||
| FDW190HK | MATa, las17Δ∷HISMX6, ARP3, his3-Δ200, LEU2, TRP1, ura3-52, GAL2 | This study |
| MATa, las17Δ∷HISMX6, ARP3-EGFP∶kanMX6, his3-Δ200, LEU2, TRP1, ura3-52, GAL2 | ||
| W303 | MATa, his3-Δ1, leu2-3,112, trp1-289, ura3-52 | P. Philippsen |
| MATa, his3-Δ1, leu2-3,112, trp1-289, ura3-52 | EUROFAN strain | |
| Y187 | MATa, gal4Δ, gal80Δ, his3, trp1-901, ade2-101, leu2-3,112, ura3-52, URA3∷GAL1(UAS)-lacZ, | Durfee et al. (1993) |
| transformed by the FRYL yeast DNA genomic library | James et al. (1996) | |
| Y190 | MATa, gal4Δ gal80Δ his3 trp-901 ade2-101 leu2-3,112 ura3-52 URA3∷GAL1(UAS)-lacZ | James et al. (1996) |
| LYS2∷GAL1(UAS)-HIS3 cyhr | ||
| CG1945 | MATa, gal4-542, gal80-538, his3, trp-901, ade2-101, leu2-3,112, ura3-52, URA3∷GAL4(3x17mers)CYC1(TATA)-lacZ,LYS2∷GAL1(UAS)-GAL1(TATA)-HIS3, cyhr | P. Legrain Froment-Racine et al. (1997) |
YMW strains and the RH strain are isogenic derivatives of YPH strains. Strain names beginning with F are isogenic FY1679 derivatives. Both of these series of strains are derivatives of S288C.