Table 3.
Sythetic lethality of las17Δ with ARP2 and ARP3 mutant alleles
| Cross | No. tetrads | No. spores Ura3+kanR | No. spores Leu2+kanR | No. viable spores | No. dead spores |
|---|---|---|---|---|---|
| las17Δ × arp2-1 | 35 | 28 | 0 | 28 | |
| las17Δ × arp2-2 | 18 | 15 | 0 | 15 | |
| las17Δ × arp2-3 | 17 | 19 | 0 | 19 | |
| las17Δ × arp2-4 | 17 | 16 | 0 | 16 | |
| las17Δ × arp2-5 | 17 | 15 | 0 | 15 | |
| las17Δ × arp2-6 | 15 | 12 | 0 | 12 | |
| las17Δ × arp2-7 | 15 | 14 | 14 | 0 | |
| las17Δ × ARP2 | 17 | 13 | 12 | 1 | |
| las17Δ × arp3-14 | 44 | 37 | 0 | 37 | |
| las17Δ × ARP3 | 45 | 53 | 48 | 5 |
Strain YMW172K (MATαlas17 ) was crossed with strains YMW201U (ARP2∶URA3) and YMW211U to YMW271U (arp2ts∶URA3). Strain YMW172K (MATα las17 Δ∷kanMX4) was crossed with strains YMW311L (MATa ARP3∶LEU2) and YMW321L (MATa arp3-14∶LEU2). Diploids were sporulated, tetrads were dissected, and viable spores were tested for G418 resistance and for growth on SC − Ura at 25°C. The genotype of dead spores was inferred from that of viable spores in each tetrad tested. For each cross the number of tetrads was analyzed, and the number of double mutants and the number of viable and dead spores among these double mutants is indicated.