Table 4.
Interactions of two-hybrid fusion proteins with Las17p and actin baits
| GAL4-AD (activation domain) | GAL4-BD (DNA binding domain) β-galactosidase activity (Miller units)
|
|
|---|---|---|
| Las17p1–643 (pBMW172) | Act1p1–375 (pDAB7) | |
| Vector (pACTII) | 00.1 ± 0.02 | 0.2 ± 0.06 |
| Act1p−24–375 (pAIP70) | 3.5 ± 0.5 | 1.4 ± 0.07 |
| Arp2p1–391 (pAMW253) | ≤0.1 | ≤0.2 |
| Arp3p1–448 (pAMW315) | ≤0.1 | ≤0.2 |
| Las17p1–643 (pAMW173) | 1.0 ± 0.1 | 1.2 ± 0.3 |
| Act1p−28–375 (2×) | 2.0 ± 0.1 | 1.1 ± 0.1 |
| Rvs167p69–482 (2×) | 50 ± 1 | 1.0 ± 0.1 |
| Rvs167p191–482 (pAIP38) | 54 ± 5 | 0.7 ± 0.2 |
| Rvs167p239–482 (2×) | 122 ± 3 | ≤0.2 |
| Rvs167p400–482 (2×) | 16 ± 2 | ≤0.2 |
| Vrp1p492–818 (2×)* | 62 ± 9 | ≤0.2 |
| Vrp1p783–818 (1×)* | 29 ± 7 | ≤0.2 |
| Lsb1p8–241 (37×) | 58 ± 5 | ≤0.2 |
| Lsb2p3–215 (9×) | 96 ± 11 | ≤0.2 |
| Lsb3p186–451 (8×) | 15 ± 4 | ≤0.2 |
| Ysc84p/Lsb4p270–468 (10×) | 6 ± 0.2 | ≤0.2 |
| Lsb5p40–213 (11×) | 34 ± 1 | ≤0.2 |
| Lsb6p67–607 (5×) | 8 ± 1 | ≤0.2 |
Vectors encoding fusions with the GAL4-(AD) were transformed into Y190 cells expressing GAL4-DB fusions with Las17p (pBMW172) or Act1p (pDAB7) and plated on SC − Trp and Leu plates. At least three colonies from each transformation were cultivated overnight in liquid selective medium (SC − Trp and Leu). β-Galactosidase activities were measured as described in MATERIALS AND METHODS. Values shown ± SD. Most double transformants that did not show blue color in the filter test were considered as approximately equal (≤) to background and were not assayed in liquid cultures. Plasmids marked with an asterisk were found in the FYRL genomic library; nonmarked entries are from the White cDNA library. Redundancy of the AD plasmids found is shown in parentheses. Not all candidates found are shown; interactions of the strongest and shortest fusions of each candidate are shown; longest fusions for Rvs167p and Vrp1p are also shown.