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Comparison of c (solid circles) and z (open circles) fragment ions from ECD mass spectra of ubiquitin 8+ transmitted through the FAIMS device at −4.7 (positive offset in the charge axis) and −6.3 V (negative offset in the charge axis). Fragment ions are plotted as the position of backbone cleavage versus fragment charge state. Ubiquitin 8+ ions are produced by ESI of 10−5 M bovine ubiquitin with 49.5/49.5/1, by volume water/methanol/acetic acid.
