Table 1.

Electron capture and fragmentation efficiency for each ubiquitin charge state at two different CV values. Electron capture efficiencies are for the first electron capture event. Electron capture and fragmentation efficiencies for different conformers of the same charge state are directly comparable; the values for different charge states are not due to slightly different experimental parameters, especially isolation waveforms.

Charge	CV (V)	Cross sectiona	Capture efficiency	Fragmentation efficiency
4	−5.3	b	0.46 ± 0.03	0 ± 0
4	−3.5	b	0.46 ± 0.03	0 ± 0
5	−6.2	940d	0.29 ± 0.04	0.10 ± 0.013
5	−5.4	960	0.46 ± 0.12	0.16 ± 0.05
6	−7.3	990c	0.66 ± 0.12	0.46 ± 0.09
6	−6.6	990c	0.67 ± 0.12	0.42 ± 0.12
7	−7.7	1000	0.35 ± 0.16	0.31 ± 0.16
7	−6.1	1235	0.65 ± 0.15	0.62 ± 0.14
8	−6.3	1330	0.901 ± 0.004	0.782 ± 0.008
8	−4.7	1585	0.832 ± 0.007	0.797 ± 0.010
9	−5.4	1540d	0.52 ± 0.19	0.43 ± 0.16
9	−4.4	1625c	0.88 ± 0.04	0.84 ± 0.02
10	−6.0	1580	0.86 ± 0.04	0.72 ± 0.05
10	−5.0	1710	0.91 ± 0.06	0.84 ± 0.06
11	−5.7	1755c	0.90 ± 0.08	0.82 ± 0.07
11	−4.8	1770	0.94 ± 0.06	0.91 ± 0.06
12	−4.9	1890	0.66 ± 0.24	0.57 ± 0.27
12	−4.3	1905	0.88 ± 0.02	0.79 ± 0.05

^aCross Section measurements as reported by Purves et al. [40].

^bPurves et al. did not report cross section measurements for ubiquitin 4+.

^cCV values used for ECD spectra are outside reported collisional cross sections [40]. Cross sections have been estimated as the nearest measured collisional cross section.

^dCV value used for ECD spectra is not within 0.25 V of the CV value used by Purves et al. when reporting collisional cross sections [40]. The cross section value in the table has been estimated by linear interpolation between reported values.