Skip to main content
. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: J Immunol. 2008 Oct 1;181(7):4613–4620. doi: 10.4049/jimmunol.181.7.4613

Figure 3.

Figure 3

Upon in vitro mitogenic stimulation, C57BL/6 derived T cells dramatically up-regulate CXCR3 while those of BALB/c mice do not. Briefly, T cells purified from uninfected BALB/c and C57BL/6 mice were stimulated in vitro by plate-bound anti-CD3/anti-CD28 antibodies for 48 hours prior to removal from stimulation and a 24 hour rest period. Flow cytometric staining of these activated cells revealed that C57BL/6 derived T cells (solid peak) but not BALB/c T cells (hollow gray peak) stained intensely for surface CXCR3 (A). Isotype controls were denoted by dotted hollow peaks. Real Time-PCR measurement of CXCR3 mRNA showed that this trend extended to the transcript level since C57BL/6 and BALB/c derived T cells induced CXCR3 mRNA at comparable levels (B). In panel A, a representative result from one of five trials is shown. Panel B represents the averaged result of three independent trials (+/−SEM). *= a P value < 0.05.