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. 1982 Sep;43(3):1118–1122. doi: 10.1128/jvi.43.3.1118-1122.1982

Characterization of a cell culture persistently infected with the DA strain of Theiler's murine encephalomyelitis virus.

R P Roos, O C Richards, J Green, E Ehrenfeld
PMCID: PMC256224  PMID: 6292455

Abstract

We established a persistent infection in L 929 cells with the DA strain of Theiler's murine encephalomyelitis virus. Our studies showed that only a small number of cells in the cultures contained infectious virus or viral antigen. A role for interferon in the maintenance of persistence was suggested. Viral isolates from the cultures were not temperature sensitive, nor did they contain viral capsid polypeptide mutations or defective interfering particles. T1 oligonucleotide maps showed evidence of mutation in two of three isolates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ACKERMANN W. W., KURTZ H. Observations concerning a persisting infection of HeLa cells with poliomyelitis virus. J Exp Med. 1955 Nov 1;102(5):555–565. doi: 10.1084/jem.102.5.555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Alwine J. C., Kemp D. J., Stark G. R. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350–5354. doi: 10.1073/pnas.74.12.5350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Anderson K. P., Klessig D. F. Synthesis of human adenovirus early RNA species is similar in productive and abortive infections of monkey and human cells. J Virol. 1982 May;42(2):748–754. doi: 10.1128/jvi.42.2.748-754.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Armstrong J. A. Semi-micro, dye-binding assay for rabbit interferon. Appl Microbiol. 1971 Apr;21(4):723–725. doi: 10.1128/am.21.4.723-725.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Batt-Humphries S., Simonsen C., Ehrenfeld E. Full-length viral RNA synthesized in vitro by vesicular stomatitis virus-infected HeLa cell extracts. Virology. 1979 Jul 15;96(1):88–99. doi: 10.1016/0042-6822(79)90175-2. [DOI] [PubMed] [Google Scholar]
  6. Brahic M., Stroop W. G., Baringer J. R. Theiler's virus persists in glial cells during demyelinating disease. Cell. 1981 Oct;26(1 Pt 1):123–128. doi: 10.1016/0092-8674(81)90040-4. [DOI] [PubMed] [Google Scholar]
  7. CROWELL R. L., SYVERTON J. T. The mammalian cell-virus relationship. VI. Sustained infection of HeLa cells by Coxsackie B3 virus and effect on superinfection. J Exp Med. 1961 Feb 1;113:419–435. doi: 10.1084/jem.113.2.419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Carp R. I. Persistent infection of human lymphoid cells with poliovirus and development of temperature-sensitive mutants. Intervirology. 1981;15(1):49–56. doi: 10.1159/000149214. [DOI] [PubMed] [Google Scholar]
  9. DANIELS J. B., PAPPENHEIMER A. M., RICHARDSON S. Observations on encephalomyelitis of mice (DA strain). J Exp Med. 1952 Dec;96(6):517–530. doi: 10.1084/jem.96.6.517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Etchison D., Ehrenfeld E. Comparison of replication complexes synthesizing poliovirus RNA. Virology. 1981 May;111(1):33–46. doi: 10.1016/0042-6822(81)90651-6. [DOI] [PubMed] [Google Scholar]
  11. HO M., ENDERS J. F. Further studies on an inhibitor of viral activity appearing in infected cell cultures and its role in chronic viral infections. Virology. 1959 Nov;9:446–477. doi: 10.1016/0042-6822(59)90135-7. [DOI] [PubMed] [Google Scholar]
  12. Inglot A. D., Albin M., Chudzio T. Persistent infection of mouse cells with Sindbis virus: role of virulence of strains, auto-interfering particles and interferon. J Gen Virol. 1973 Jul;20(1):105–110. doi: 10.1099/0022-1317-20-1-105. [DOI] [PubMed] [Google Scholar]
  13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  14. Lehrich J. R., Arnason B. G., Hochberg F. H. Demyelinative myelopathy in mice induced by the DA virus. J Neurol Sci. 1976 Oct;29(2-4):149–160. doi: 10.1016/0022-510x(76)90167-2. [DOI] [PubMed] [Google Scholar]
  15. Lipton H. L., Friedmann A. Purification of Theiler's murine encephalomyelitis virus and analysis of the structural virion polypeptides: correlation of the polypeptide profile with virulence. J Virol. 1980 Mar;33(3):1165–1172. doi: 10.1128/jvi.33.3.1165-1172.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lipton H. L., Gonzalez-Scarano F. Central nervous system immunity in mice infected with theiler's virus. I. Local neutralizing antibody response. J Infect Dis. 1978 Feb;137(2):145–151. doi: 10.1093/infdis/137.2.145. [DOI] [PubMed] [Google Scholar]
  17. Lipton H. L. Persistent Theiler's murine encephalomyelitis virus infection in mice depends on plaque size. J Gen Virol. 1980 Jan;46(1):169–177. doi: 10.1099/0022-1317-46-1-169. [DOI] [PubMed] [Google Scholar]
  18. Lipton H. L. Theiler's virus infection in mice: an unusual biphasic disease process leading to demyelination. Infect Immun. 1975 May;11(5):1147–1155. doi: 10.1128/iai.11.5.1147-1155.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nelson-Rees W. A., Hooser L. E., Hackett A. J. Chronic poliovirus infection of cocultivated monkey cells harboring the Mason-Pfizer monkey virus. J Natl Cancer Inst. 1972 Sep;49(3):713–725. [PubMed] [Google Scholar]
  20. Penney J. B., Jr, Wolinsky J. S. Neuronal and oligodendroglial infection by the WW strain of Theiler's virus. Lab Invest. 1979 Mar;40(3):324–330. [PubMed] [Google Scholar]
  21. Rubinstein S., Familletti P. C., Pestka S. Convenient assay for interferons. J Virol. 1981 Feb;37(2):755–758. doi: 10.1128/jvi.37.2.755-758.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. TAKEMOTO K. K., HABEL K. Virus-cell relationship in a carrier culture of HeLa cells and Coxsackie A9 virus. Virology. 1959 Jan;7(1):28–44. doi: 10.1016/0042-6822(59)90175-8. [DOI] [PubMed] [Google Scholar]
  23. Wroblewska Z., Gilden D. H., Wellish M., Rorke L. B., Warren K. G., Wolinsky J. S. Virus-specific intracytoplasmic inclusions in mouse brain produced by a newly isolated strain of Theiler virus. I. Virologic and morphologic studies. Lab Invest. 1977 Dec;37(6):595–602. [PubMed] [Google Scholar]

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