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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Biomaterials. 2008 Aug 20;29(32):4292–4299. doi: 10.1016/j.biomaterials.2008.07.018

Fig. 1.

Fig. 1

Schematic of experimental design. Study 1: Gel: single-phase chondrocyte-seeded agarose constructs serve as controls, OC(bone): multi-phase osteochondral constructs formed with a trabecular bone substrate, Co-culture(bone): single-phase gel cultured adjacent to bone to test for the effect of soluble factors, Co-culture(demin bone): Gel constructs cultured adjacent to demineralized bone to control for the effect of soluble minerals, No TGF-β3: Gel constructs cultured in medium without TGF-β3 to test for possible decrease in availability of growth factors through absorption into bone. Study 2: Bone is introduced to gel constructs at day 14 (Bone Introduced on day 14) or at day 28 (Bone Introduced on day 28) to examine the possibility of forming osteochondral constructs after gel is more developed. Study 3: OC(Tantalum): Multi-Phase osteochondral constructs formed with tantalum substrate, Gel and OC(bone) serve as controls. Each study was carried out separately and all groups were cultured for 42 days.