Figure 6.

PTX abolishes the effect mediated by MCH in hypocretin/orexin neurons. A, The time course of a typical experiment showing MCH does not decrease the enhancement of the frequency of spikes induced by hypocretin-1 in hypocretin/orexin neurons in slices pretreated with PTX for 3 h. Time points at which raw traces were taken are indicated by 1–4. The dotted line represents the baseline. B, C, Sample traces of spikes recorded and pooled data from all tested neurons are presented. *p < 0.01, compared with control.