Table 3. S-(2′,4′-Dichlorophenacyl)glutathione Reductase Activity of Human GSTO1-1 Variantsa.
| Enzyme | Specific activity
(μmol GSSG formed min-1 mg protein-1) |
|---|---|
| hGSTO1-1 | 1.3 ± 0.1 |
| hGSTO1-1 ΔE155, E208 | 1.3 ± 0.05 |
| hGSTO1-1 ΔE155, K208 | 1.1 ± 0.03 |
| hGSTO1-1 K208 | 1.1 ± 0.07 |
| hGSTO1-1 D140 | 1.1 ± 0.05 |
| hGSTO1-1 A32 | <0.01 |
| rat SPG-R | 1.0 ± 0.03 |
a
The substrate was 2,2′,4′-trichloroacetophenone 2a; reaction rates were determined with Assay I (Materials and Methods). The data are expressed as means ± SD. Reported rat hepatic SPG-R activity (19) is shown for comparison.