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. Author manuscript; available in PMC: 2008 Oct 6.
Published in final edited form as: Chem Res Toxicol. 2007 Jan;20(1):149–154. doi: 10.1021/tx600305y

Table 5. Kinetic Parameters for GSTO1-1 with S-(Phenacyl)glutathione 1b and Glutathione and 2-Mercaptoethanol as Thiol Substratesa.

Glutathione 2-Mercaptoethanol
kcat
(S-1)		 KmSPG
(mM)		 kcat/Km
(M-1 S-1)		 kcat
(S-1)		 KmSPG
(mM)		 kcat/Km
(M-1 S-1)
GSTO1-1 0.8 ± 0.4 0.062 ± 0.01 1.3 × 104 5.8 ± 0.47 0.33 ± 0.05 1.76 × 104
a

Reaction rates were determined with Assay II (Materials and Methods). Data are shown as means ± SD of at least 3 observations.