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. Author manuscript; available in PMC: 2008 Oct 6.
Published in final edited form as: J Immunother. 1997 Jul;20(4):276–286. doi: 10.1097/00002371-199707000-00004

FIG. 4.

FIG. 4

Specific interferon-γ (IFN-γ) release by CD8+ T lymphocytes from six HLA-A*0201 patients with metastatic melanoma. Anti-MART-1 cytotoxic T lymphocytes (CTL) were generated by stimulation with dendritic cells (DC) pulsed with MART-127-35 peptide or infected with rV-MART-1 and tested 7–9 days after stimulation (week 1). After 7 days, anti-MART-1 CTL stimulated with peptide-pulsed DC were restimulated with peptide-pulsed DC while cultures stimulated with rV-MART-1 DC were restimulated with DC infected with non-cross-reacting rF-MART-1. These CTL cultures were retested 7 days after the restimulation (week 2). Effectors were tested against T2 cells pulsed with 1 μg/ml peptide. Anti-MART-1 CTL cultures generated with peptide-pulsed DC were tested against T2 cells pulsed with 1 μg/ml of MART-127-35 (hatched bars) and G9-209-2M (gray bars) peptides. Anti-MART-1 CTL cultures generated with rV-MART-1 DC were tested against T2 cells pulsed with 1 μg/ml MART-127-35 (black bars) and G9-209-2M (white bars) peptides.