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. Author manuscript; available in PMC: 2008 Oct 6.
Published in final edited form as: J Immunother. 1997 Jul;20(4):276–286. doi: 10.1097/00002371-199707000-00004

FIG. 5.

FIG. 5

Cytotoxic activity (calcein AM release) of anti-MART-1 cytotoxic T lymphocytes (CTL) induced with recombinant virus-infected dendritic cells (DC). Anti-MART-1 CTL were induced by a primary in vitro stimulation with DC infected with rV-MART-1 followed 7 days later with a second stimulation with DC infected with rF-MART-1 virus (rV-MART-1 = left two panels). By reversing the sequence of the viral vectors used for stimulation, anti-MART-1 CTL were also induced by a primary stimulation with DC infected with rF-MART-1 virus followed 7 days later by a second stimulation with DC infected with rV-MART-1 virus (rF-MART-1 = right two panels). Recognition of the epitope MART-127-35 was tested by pulsing T2 cells with 1 μg/ml MART-127-35 (■) or 1 μg/ml irrelevant G9-209-2M (□). Recognition of naturally processed MART-1 epitopes was tested against the 624.38 (▲) HLA-A*0201+/MART-1+ and the 624.28 (△), HLA-A*0201-/MART-1 + melanoma clones.