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. Author manuscript; available in PMC: 2008 Oct 6.
Published in final edited form as: Development. 2008 Apr 23;135(11):2043–2053. doi: 10.1242/dev.015818

Figure 1. Generation of the talin 1 conditional allele.

Figure 1

(A) Scheme of the targeting strategy. Homologous recombination of the targeting construct into the Tln1 gene introduced a loxP site (triangle) downstream of coding exon 5 and a floxed pgk-neo cassette upstream of the first coding exon (exon 2). CRE expression induced three recombination events. Type II deletion: removal of the Neo cassette (Tln1 fl/fl); Type III deletion: removal of exons 1−4 (not shown); Type I deletion: removal of pgk-neo and exons 1−4 (Tln1−). a-c denote primers for PCR reactions shown in (B). (B) Recombination pattern observed by PCR on DNA extracted from E14.5 and P2 wild-type (WT) and mutant (MT) littermates. Upper panel: A 707 bp band indicative of the recombined allele (Rec) was observed in samples from Tln1HSA-CREko mice. Middle panel: the presence of floxed (fl) and wild type (wt) alleles was confirmed; Lower panel: the presence of CRE recombinase (CRE) was confirmed.