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. 2008 Sep 18;8:74. doi: 10.1186/1472-6750-8-74

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Copyright © 2008 Cai et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The map of pSi4.1-siFAK phagemid particle and analysis of the ssDNA released from phagemid particles. A: pSi4.1-siFAK phagemid particle was constructed as follows: the shRNA against FAK was inserted into the modified pSilencer4.1 vector digested with BamHI and HindIII. B: ssDNA was purified from phagemid particles to analyze the ratio of phagemids to helper phage genomes packaged in the phagemid particles. The results indicated that almost all the DNA packaged comprised phagemids. Lane M: λ DNA/HindIII; Lane 1: pSi4.1-siFAK phagemid particles; Lane 2: pSi4.1-simock phagemid particles.