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Northern blot analysis. Northern blot analysis of total RNA isolated from E. coli DH5α pOSM12 at 1% and 3% NaCl (A) and from E. coli DH5α pOSM2 at 3% NaCl (B) in minimal medium MM63 was performed with specific RNA probes for each of the ectoine genes ectA, ectB and ectC (0.4 kb = ectC, 0.6 kb = ectA, 1.3 kb = ectB, 1.8 kb = ectBC, 2.0 kb = ectAB). Arrows indicate where mRNA bands matching the calculated size of gene should be located. (+) and (-) refer to presence and absence of IPTG.
