Figure 3.

Transcription initiation sites and putative promoter regions. Transcription initiation sites and positions of putative σ^A, σ^B- and σ⁷⁰/σ^S-dependent promoters upstream of ectA (A), ectB (B) and ectC (C). The -35 and -10 regions are underlined and the start codons ATG are framed. The transcription initiation sites as determined by RACE are typed bold, underlined twice and marked (+1). The DNA sequence upstream ectA which is deleted in pOSM16 (see text) is underlayed grey. The Sau3A restriction site used for the construction of pOSM2 (↱pOSM2) is marked. |◀◀: last nucleotide of the cDNA fragment from RACE experiment, which was terminated 89 bp upstream of the start codon of ectA (for E. coli) and 83 bp upstream of ectC (for M. halophilus) (see text).